Universidad Peruana Cayetano Heredia
Departamento de Bioquímica, Biología Molecular y Farmacia

Genética y Genómica Avanzada.  2007-I

Profesor: Gisella Orjeda

Creditaje: (3 horas crédito)

Aula 2EB

Horario de clases
Inicio de clases: Lunes 12 de Marzo

Descargar syllabus

PDFs

 


El secuenciamiento de genomas

Gisella Orjeda

 

Lunes

12/03/2007

Introduccion al curso y conceptos importantes

 

 

Miercoles

14/03/2007

1.The International Human Genome Mapping Consortium.  Nature. 2001.  409, 934-941A physical map of the human genome.

Grace Velapatiño-Julio Aguilar

http://www.nature.com/nature/journal/v409/n6822/full/409934a0.html

Lunes

19/03/2007

2. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana.

Nature 2000.  408, 798-815

Roberto Lozano,Carlos Balmaceda

pdf

Comparacion de genomas y uso de secuencia

 

 

Miercoles

21/03/2007

3.The Chimpanzee Sequencing and Analysis Consortium. Nature. 2005. Initial sequence of the chimpanzee genome and comparison with the human genome

Sebastian Gonzales-Silvia Davila

http://www.nature.com/nature/journal/v437/n7055/full/nature04072.html

Lunes

26/03/2007

4.M A Campbell, B J Haas, J P Hamilton, S. Mount,and C Robin Buell. Comprehensive analysis of alternative splicing in rice and comparative analyses with Arabidopsis. BMC Genomics. 2006; 7: 327

Presentacion1 Presentacion2 Presentacion3

Felipe Yon - Carlos Castrillon

 

http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=17194304

Miercoles

28/03/2007

8 am Videoconferencia Xose Fernandez.  EBI.  Introduccion a Ensembl y  BioMart, herramienta de minería de datos

 

 

www.ensembl.org

Lunes

02/04/2007

Examen 1

 

 

 

 

 

 

 

Tecnicas para secuenciamiento  y analisis de genomas

Gisella Orjeda

 

Miercoles

04/04/2007

5.Osoegawa. 2002 Genome research .A Bacterial Artificial Chromosome Library for Sequencing the Complete Human Genome (este articulo pertenece al tema Tecnicas…)

Jhuliana Amaro-Maria Julia Gonzales

http://www.genome.org/cgi/content/full/11/3/483

6.A. Marra et al. High Throughput Fingerprint Analysis of Large-Insert Clones.Marco Genome Research Vol. 7, Issue 11, 1072-1084, November 1997

Irene Delgado de la Flor- Oscar Aguinaga

http://www.genome.org/cgi/content/full/7/11/1072?ijkey=288633803f769690c0d31ca1be706649d698e569

Lunes

09/04/2007

7.Chen et al.An Integrated Physical and Genetic Map of the Rice Genome.The Plant Cell, Vol. 14, 537-545, March 2002

Grace Velapatiño-Julio Aguilar

http://www.plantcell.org/cgi/content/full/14/3/537

Miercoles

11/04/2007

8.Cheng. 2001.High-Resolution Pachytene Chromosome Mapping of Bacterial Artificial Chromosomes Anchored by Genetic Markers Reveals the Centromere Location and the Distribution of Genetic Recombination Along Chromosome 10 of Rice. Genetics.Vol. 157, 1749-1757

Sebastian Gonzales-Silvia Davila

http://www.genetics.org/cgi/content/full/157/4/1749?ijkey=a502e8706e8f1ef457eaecd2a815caced98d40d4

9. Brent. Genome annotation past, present, and future: How to define an ORF at each locus.Genome Research 15:1777-1786, 2005

Luis Castillo-Cristina Rojas

http://www.genome.org/cgi/content/full/15/12/1777

Lunes

16/04/2007

Examen 2

 

 

Genomica funcional

 

 

 

Miercoles

18/04/2007

10. Uetz et al 2000. A comprehensive analysis of protein–protein interactions in Saccharomyces cerevisiae. Nature 403, 623-627

Jhuliana Amaro-Maria Julia Gonzales

http://www.nature.com/nature/journal/v403/n6770/full/403623a0.html

11.Hugues Roest Crollius et al. Nature Genet. 2000 Estimate of human gene number provided by genome-wide analysis using Tetraodon Nigroviridis DNA sequence

Roberto Lozano,Carlos Balmaceda

http://www.nature.com/ng/journal/v25/n2/full/ng0600_235.html

Lunes

23/04/2007

12.Genome Research 2005. Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species. The Rice Chromosome 3 Sequencing Consortium.                                                        

Felipe Yon - Carlos Castrillon

http://www.genome.org/cgi/content/full/15/9/1284

 

13. Zhu&Buell. 2007 Improvement of whole-genome annotation of cereals through comparative analyses.Genome Research. published online Feb 6, 2007.

Sebastian Gonzales-Silvia Davila

http://www.genome.org/cgi/doi/10.1101/gr.5881808

Miercoles

25/04/2007

8am: Videoconference-Insights into the Rice Genome.  Dr. Robin Buell. Associate Investigator. TIGR

 

www.tigr.org/faculty/Buell_Robin/index.shtml

Lunes

30/04/2007

feriado

 

 

Miercoles

02/05/2007

8am:  Videoconference- Comparative Genomics in Vertebrates: Lessons from the Tetraodon GenomeHugues Roest Crollius, Ecole National Superieure, Paris France (1h)

9am: Videoconference: La complejidad de los genes humanos, Roderic Guigo, Centre de Regulacio Genomica, Grup de Recerca en Informatica Biomedica (IMIM and UPF), Barcelona, España (1h)

 

http://www.biologie.ens.fr/dyogen/?lang=en

 

http://genome.imim.es

 

Lunes

07/05/2007

14. Bachem et al. 2002 (Chapter 3.3.3) CDNA y AFLP y Brugmans 2002.                                                                     

Roberto Lozano,Carlos Balmaceda

 

 

15. A novel method for the construction of genome wide transcriptome maps.  The Plant Journal (2002) 31(2), 211±222 (leer resto de articulos de Bachem)

Felipe Yon - Carlos Castrillon

 

Miercoles

09/05/2007

Videoconferencia- Dr. Christian Bachem Wageningen university.Holanda. Principios de transcriptomica y usos de cDNA and chip para estudiar desarrollo y calidad en Solanum

 

http://www.dpw.wau.nl/pv/staff/staffmember.php?email=Christian.Bachem@wur.nl

 

 

 

 

 

Lunes

14/05/2007

Examen 3

 

 

T-DNA Mutagenesis y transcriptomica

 

 

Miercoles

16/05/2007

 T-DNA Mutagenesis: From Tagging to Insertion Sequence Databases

Luis Destefano

slides clase

Lunes

21/05/2007

16. Floral dip: a simplified method forAgrobacterium-mediated transformation of Arabidopsis thaliana, (1998) Plant Journal, 16-735-743

Irene Delgado de la Flor - Oscar Aguinaga

 

17. Large-scale T-DNA mutagenesis in Arabidopsis for functional genomic analysis. (2000) Functional Integrative Genomics 1:25-34

Luis Castillo - Cristina Rojas

 

Miercoles

23/05/2007

18. T-DNA insertational mutagenesis for functional genomics in rice. (2000) Plant Journal, 22: 561-570.

Maria Julia Gonzales - Jhuliana Amaro

 

19. Agrobacterium T-DNA integration in Arabidopsis is correlated with DNA sequence compositions that occur frequently in gene promoter regions. (2005) Functional Integrative Genomics 5: 240-253.

Roberto Lozano - Carlos Balmaceda

 

Lunes

28/05/2007

Transcriptomics in Plants: From Expression to Gene Function

Luis Destefano

slides clase

Miercoles

30/05/2007

20. Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays (2000) Nature Biotechnology 18: 630-634

Irene Delgado de la Flor - Oscar Aguinaga

 

21. Design issues for cDNA microarrays experiments (2002) Nature Reviews Genetics, 3: 579-588

Felipe Yon - Carlos Castrillon

 

Lunes

04/06/2007

22. Fundamentals of Experimenatl Design for cDNA microarrays. (2002)  Nature Genetics, 32: 490-495.

Sebastian Gonzales - Silvia Davila

 

23. Expression profiling using a hexamer-based universal microarray (2004) Nature Biotechnology, 22: 418-426.

Grace Velapatiño-Julio Aguilar

 

24.Gene expression profiling of potato responses to cold, heat, and salt stress (2005) Functional Integrative Genomics 5: 201-207

Maria Julia Gonzales - Jhuliana Amaro

 

Genetica hoy en dia en los programas de mejoramiento

 

 

Miercoles

18/06/2007

6pm Videoconferencia Augusto Becerra.CSIRO-australia. QTL mapping Marker assisted selection in Cotton

 

 

Lunes

20/06/2007

Examen 4

 

 




Última modificación: 13 de Mayo, 2007