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Sumario
§ Personal
del IMT en entrenamiento en el Extranjero
Nombre:
Berónica Infante García
Posición:
Marie Curie Scholarship Recipient.
Proyecto:
"Towards controlling antimicrobial use and resistance in low-income countries -
an intervention study in Latin America" (ANTRES) is currently ongoing in Peru
and Bolivia
El
objetivo general del proyecto ANTRES es desarrollar un marco de trabajo
conceptual describiendo el uso y resistencia de antimicrobianos en bacterias
patógenas y los factores que influyen en niňos de 6 a 72 meses de edad en
comunidades urbanas y rurales remotas en Bolivia y Perú. Se desarrollará y
evaluará una estrategia de intervención que involucra servicios de salud locales
dirigidos al público en general con respecto al uso de antimicrobianos y
tendencias de resistencia bacterial comparando un grupo intervenido con un
grupo control, basados en los resultados.
Institución:
Laboratorio de Microbiología Clínica – MTC, Instituto Karolinska Supervisor:
Göran Kronvall, MD, PhD
País:
Estocolmo,
Suecia
Periodo:
del 18th
Octubre del 2002 al 8th de Abril del 2003.
Entrenamiento:
Microbiología Clínica:
Pruebas de susceptibilidad
antimicrobial; método de difusión en placa (www.srga.org),
Metodos de identificacion bacterial.
Biología Molecular:
Reacción en cadena de la
polymerasa (PCR) y Sequenciamiento .
Análisis de las mutaciones
presentes en la región
determinante resistente a quinolonas (QRDR), siendo la mas importante la región
girasa A (gyrA).
Seguimiento de
Integrones portadores de genes cassettes resistentes a diferentes antibioticos.
Reportes :
"Mutations in gyrA in
quinolone resistant isolates of Escherichia coli from Bolivia and Peru"
" Incidence and Characterization of drug resistance gene cassettes associated
with Integrons in healthy children isolates of Escherichia coli from Bolivia and
Peru, during the pilot study"
Project:
"Towards controlling antimicrobial use and resistance in low-income countries -
an intervention study in Latin America" (ANTRES) is currently ongoing in Peru
and Bolivia
The general
objective of the ANTRES project is to develop a conceptual framework describing
antimicrobial use and antimicrobial resistance in bacterial pathogens and the
influencing factors in children aged 6 to 72 months in urban and remote rural
communities in Bolivia and Peru. Based on the findings, an intervention strategy
involving local health services addressing the general public will be developed
and evaluated regarding antimicrobial use and bacterial resistance trends in an
intervention group compared to a control group.
Institution:
Clinical Microbiology – MTC, Karolinska Institute
Supervisor:
Göran
Kronvall, MD, PhD
Country :
Stockholm, Sweden
Period:
From
October 18th 2002 to April 8th 2003.
Training:
Clinical
Microbiology,
Antimicrobial Susceptibility Testing (AST) (www.srga.org),
Bacterial Species Identification
Molecular
Biology,
PCR amplification and DNA sequencing and analysis of the gyrA region and
integrons.
Reports :
"Mutations in gyrA in
quinolone resistant isolates of Escherichia coli from Bolivia and Peru"
" Incidence and Characterization of drug resistance gene cassettes associated
with Integrons in healthy children isolates of Escherichia coli from Bolivia and
Peru, during the pilot study"
Nombre:
MSc. Ivan Best Cuba
Posición actual:
Responsable del
Laboratorio de Inmunologia (IMTAvH - UPCH)
Institución de
Prince Leopold
Institute of Tropical Medicine, Amberes.
Entrenamiento:
Colaboración Institucional IMTAvH-IMTAP (DGIS2)
Supervisor:
PhD. Guido Vanham
País:
Bélgica
Período:
15 Enero – 1 Mayo
2003
Proyecto:
“Caracterización de la
respuesta immune celular en pacientes infectados con HTLV-1 durante la
ocurrencia de infecciones oportunistas”
El objetivo del presente
proyecto es evaluar como la infección con HTLV-1 afecta la respuesta inmune
celular especifíca predisponiendo a la ocurrencia de infecciones oportunistas,
tales como la Strongyloidasis. Durante el estudio, se caracterizará las sub-poblaciones
de células Th1 y Th2 de pacientes infectados con HTLV-1 de las zonas altoandinas
del Perú con presencia o no de Strongyloidasis.
Entrenamiento
Citometría de
flujo:
·
Estandarización de los métodos TruCount y FlowCount para la determinación del
número absoluto de células T CD4+ en sangre periférica.
Técnicas
inmunológicas:
·
Cultivo in
vitro de células mononucleares de sangre periférica (PBMC).
·
Proliferación
de células T a partir de células mononucleares de sangre periférica.
·
Evaluación de
la respuesta inmune celular (Th1 y Th2) mediante la medición por ELISA de las
citocinas: IFN-g,
TNF-a,
IL-2, IL-4, IL-10, IL-12p70 y IL-15.
Biología
Molecular:
·
PCR
diagnóstico de PTLV, HTLV-1 y HTLV-2.
·
Nested PCR
·
Secuenciamiento de la región LTR y env del virus HTLV-1
Actual
Position:
Responsible of
Immunology Lab (IMTAvH - UPCH)
Training
Institution:
Prince Leopold Institute of Tropical Medicine, Amberes.
Institutional collaboration
IMTAvH-IMTAP (DGIS2)
Supervisor:
PhD. Guido Vanham
Country:
Belgium
Period:
15th January – 1st May 2003
Project: “Characterization
of cellular immune response in HTLV-1 infected patients during the occurrence of
opportunistic infections”
The objective of the present project is evaluated how the infection with HTLV-1
affects the specific cellular immune response predisposes the occurrence of
opportunistic infections, such as the Strongyloidaisis. During this study, the
Th1 and Th2 subpopulations will be characterized in HTLV-1 infected patients of
the high andean zones of Peru that shown or not Strongyloidasis.
Training:
Flow cytometry
Standardization
of TruCount and FlowCount methods for the determination of CD4+ T cells absolute
number from peripheral blood.
Immunological
Techniques
·
In vitro
culture of peripheral blood mononuclear cells (PBMC).
·
T
cell proliferation from peripheral blood mononuclear cells.
·
Evaluation of the cellular immune response (Th1 and Th2) by ELISA of following
cytokines: IFN -g,
TNF-a,
IL-2, IL-4, IL-10, IL-12p70 and IL-15.
Molecular
Biology
·
PCR Diagnostic of PTLV, HTLV-1 y HTLV-2.
·
Nested PCR
·
Sequencing of LTR and env region from HTLV-1 virus.
PUBLICACIONES
2003
REVISTAS EXTRANJERAS
1. Meyer, W ; Castañeda, A ; Jackson, S ; Huynh, M ; Castañeda, E and the
IberoAmerican Cryptococcal Study Group (Peru: Bustamante, B
- Inst Med Trop AvH-UPCH)
Molecular Typing of IberoAmerican Cryptococcus neoformans Isolates
-- Emerg Infect Dis 9 (2): 189-195, February 2003.
A network was
established to acquire basic knowledge of Cryptococcus neoformans in
IberoAmerican countries. To this effect, 340 clinical, veterinary, and
environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru,
Venezuela, Guatemala, and Spain were typed by using M13 polymerase chain
reaction-fingerprinting and orotidine monophosphate pyrophosphorylase (URA5)
gene restriction fragment length polymorphsm analysis with HhaI and Sau96I in a
double digest. Both techniques grouped all isolates into eight previously
established molecular types. The majority of the isolates, 68.2% (n=232), were
VNI (var. grubii, serotype A), which accords with the fact that this
variety causes most human cryptococcal infections worldwide. A smaller
proportion, 5.6% (n=19), were VNII (var. grubii, serotype A); 4.1%
(n=14), VNIII (AD hybrid), with 9 isolates having a polymorphism in the URA5
gene; 1.8% (n=6), VNIV (var. neoformans, serotype D); 3.5% (n=12), VGI;
6.2% (n=21), VGII; 9.1% (n=31), VGIII, and 1.5% (n=5) VGIV, with all four VG
types containing var. gatii serotypes B and C isolates.
2. Godoy
P ; Tiraboschi N ; Severo L ; Bustamante B ; Calvo B ; Almeida de P ; Matta da A
; Colombo A
Species distribution and
antifungal susceptibility profile of Candida spp bloodstream isolates from Latin
American hospitals. -- Mem Inst Oswaldo Cruz, Río de Janeiro 98:1-5, 2003.
3.
Sanchez
J, Campos PE, Courtois B, Gutierrez L, Carrillo C, Alarcon J, Gotuzzo E, Hughes
J, Watts D, Hillier SL, Buchanan K, Holmes KK.
Prevention of
sexually transmitted diseases (STDs) in female sex workers: prospective
evaluation of condom promotion and strengthened STD services. -- Sex Transm Dis
30(4): 273-9, 2003.
In Peru, a previous study
of government-sponsored periodic examinations of female sex workers (FSWs) found
no significant impact on rates of sexually transmitted diseases (STDs). GOAL:
This study assessed the impact of technically improved periodic STD services on
STD prevalence and on consistent condom use (CCU). STUDY DESIGN: The study
involved monthly follow-up of 917 FSWs at two STD clinics, with evaluations
before and during implementation of strengthened STD services, which included
periodic screening and treatment for gonorrhea, chlamydial infection,
trichomoniasis, bacterial vaginosis (BV), and syphilis; counseling; and supply
of condoms. Outcome analyses were adjusted for participation bias. RESULTS:
During 7908 person-months of observation, the prevalences of gonorrhea,
chlamydial infection, trichomoniasis, and BV declined significantly and CCU
increased significantly, with similar trends in both clinics. During follow-up,
gonorrhea, chlamydial infection, trichomoniasis, and BV were negatively
associated with follow-up after screening. BV also was positively associated
with use of an intrauterine device and negatively with douching. CCU during
follow-up was associated with significantly decreased risk of gonorrhea,
chlamydial infection, and trichomoniasis. CONCLUSIONS: Strengthened periodic
screening for and treatment of confirmed STD, in addition to condom promotion
and provision, represent feasible, effective interventions in commercial sex,
and time series analyses can provide a useful approach to evaluating new
interventions.
REVISTAS PERUANAS
1.
Pinto J ; Vidal J ; Busalleu A ; Mercado J ; Ramírez D ; Valdivia J ;
de los Ríos R
Infección por
el virus de la hepatitis C en donantes del banco de sangre en el Hospital
Nacional
Cayetano
Heredia (1998-2002)
Rev Gastroenterol
Peru 23(1): 22-8, 2003.
2.
Barahona L ; Maguiña C ; Náquira C ; Terashima A ; Tello R
Blastocystosis humana: estudio
prospectivo, sintomatología y factores epidemiológicos asociados.
Rev Gastroenterol
Peru 23(1): 29-35, 2003.
§
Maestria Internacional de
Control de Enfermedades Infecciosas y Tropicale,
se
realizará en alianza con la Facultad de Salud Pública (FASPA) de la UPCH. Tenemos el apoyo técnico y financiero del Sparkman Center . Asi mismo la asesoría de la OPS (Drs. Brito y R. Gusmao).
§
IMT Amberes : Se esta
fortaleciendo la
colaboración Institucional para el impulso de
1) Clinical Research,
2) Laboratorio de Inmunología
3) Biología Molecular
Así como otros aréas de desarrollo como AIDS y TBC,
así como Micología, Malaria y otras enfermedades locales.
.
Entrenamiento
del Personal de Enfermería del DETD.
Se
esta entrenando al personal de enfermería del Departamento de Enfermedades
Infecciosas y Tropical del HNCH
desde el mes de setiembre en el uso de las tecnologias de la información en
computo, se esta apoyando con la
Capacitación del Personal de Enfermería en el Centro de Computo del IMT-avh-UPCH.
-
CRICS 2003 - Mayo - México
Experiencia Peruana en el Uso de las Tecnologías de la
Información para los Profesionales de la Salud .
Murrugarra L, Calvo F, Gotuzzo E, Caro G, Vega J,
Aparcana J, Avalos J, Changa V, Valero F, Chavez H,
Rojas F.
Dr.
Miguel W. Tregnaghi
Dr. Daniel Stamboulian
Dr. Eduardo Gotuzzo
________________________________________________________________________________________________________
Para más información de
cualquier tipo relacionada con las
actividades del IMT-AVH-UPCH,
consultar el siguiente URL que está en continua
actualización:
Visite
el web site del IMT "AvH"- UPCH
http://www.upch.edu.pe/Tropicales
Suscripciones
de Boletin Electrónico
Poner
en subject/asunto: SUSCRIBE
ladym@upch.edu.pe
Si
no desea recibir el Boletin Electrónico
Poner en subject/asunto: CANCELAR SUSCRIPCIÓN
ladym@upch.edu.pe
Boletín
Electrónico del IMT-AvH-UPCH
Dr. Eduardo Gotuzzo -
Director
P.O. Box 4314, Lima 100 – Perú
Telf:
511-4823910, 4823903, 4823401, Fax: 511-4823404
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