A.      Positions and Honors.

Positions and Employment

1980:                      Research Assistant, Laboratory of Microbiology, Instituto Farmacologico S.A. (IFASA).

1981:                      Research Assistant, Laboratory Microbiology (LMB).

1981:                      Invited Teacher in Practices of Microbiology. Universidad Ricardo Palma.

1982:                       Researcher, National Institute of Health – Lima, Peru., Laboratory of Special Bacteriology.

1981-1987:              Head of the Laboratory of Microbiology. Laboratory of Immune-Diagnostic Cantella Colichón.

1984-1985:              Researcher, National Institute of Health – Lima, Peru. Reference Laboratory for Microbiology, Public Health.

1985-Present         Associate Researcher, Instituto de Medicina Tropical Alexander von Humboldt" Universidad Peruana Cayetano Heredia.

1985-94:                  Researcher, National Institute of Health – Lima, Peru. Address of Epidemic Investigation (before Center of Investigation Dr. Hugo Lumbreras).

1989-91: Auxiliary Professor of Microbiology. Universidad Ricardo Palma.

1989-91: Teacher, Food Microbiology. Universidad Ricardo Palma.

1991:                       Invited professor, OPS/Brasil in the Workshop, Control of Vectorial Mechanisms of Activity of Sporulated Bacterial Pathogens of Insects. Trujillo, Venezuela, Sep. 28 to Oct. 16.

1992-Present:        Invited Professor. Universidad Peruana Cayetano Heredia, Departments of Parasitology, Microbiology and Pharmacology.

1995-97:                  Researcher. National Institute of Health – Lima, Peru. Reference Laboratory for Entomology, Public Health.

1995:                      Professor, Food Microbiology. Universidad Ricardo Palma.

1996:                       Professor, Applied Entomology in Vectors of Metaxenics Diseases." Sub-region of Salud I Jaen, Program of Malaria Control or OEM. Jaen 26-31.

1995-97:                  Researcher, National Institute of Health – Lima, Peru., Entomology Laboratory,

1997- 1998              Researcher, National Institute of Health – Lima, Peru, Laboratory of Special Bacteriology.

2005- Present        Researcher  Professor, Intitu de medicina tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia.

Other Experience and Professional Memberships

2005-2006:    Member of the Directive Committee at FOPECAL (Foro Peruano de Capacitación Laboral).

2004.  -2005:   Member of  Regulate Advisory Committee of the National Sanitary Strategy of Prevention

         and Control of  Metaxenic Diseases  and other Diseases Transmitted by Vectors, NIH-Perú.

2003- 2007:    Member of the Directive Committee at Instituto de Medicina Tropical Alexander von

          Humboldt–UPCH.

2002-Present:  Peruvian Society for Immunology.

1993-2005:     Member, Peruvian society for Parasitology, Lima - Peru.

1984  –Present: Biologist College from  Peru (# CBP. 1081).

Honors

1987:       First Prize,  “Congress for Young Scientists”.  Universidad Peruana Cayetano  Heredia. Lima-Perú. “Determination of faecal contamination in Laguna Grande National Reserve”, Paracas/Pisco, Perú. 

1995:     Recognition for dedication and contribution to the world of science. On behalf of the people of

Canada . The Right  Honorable Jean. Chertien, P.C., M.P., On the 25th Anniversary of International Development Research Centre.

1996:       Parchment for dedication and contribution  in cultural advance in Vector Control. On behalf of the Salitral. Piura-Peru community, On the occasion of  the 7º ABIMSA Anniversary.

1999:       Recognition for support after hurricane Mitch. On behalf  of  The Children at our Little Brothers & Sisters Orphanage. Honduras, Central America.

2000:     Second Prize as Tutor. National Prize at research in Medicine Abeefe Bristol-Myers Squibb. Lima.Perú. “Cat scratch disease, the first prospective study in Lima, Perú”.

2000:       First Prize as Tutor. National Prize at research in Medicine Abeefe Bristol-Myers Squibb. Lima, Perú. Identification of B. bacilliformis from blood samples by PCR in Lima, Perú..

2002:       Identification of others Bartonella from blood samples by PCR in Lima, Perú. Second Prize as Tutor. National Prize at research in Medicine Abeefe Bristol-Myers Squibb. Lima.Perú.

2004:       Tutor. Stimulus prize to the investigation project Bárbara Ann Kotowsky de Tejada." To the students: Rosa María Castillo Shilder, Cyntia Terrones and David Angel Yabar Bedoya. Title: Knowledge Attitudes and practice regarding Bartonellosis in the residents of the District of Ollantaytambo, Province of Urubamba in the Sacred Valley of the Inca. Cuzco-Peru".

2005:       Medal of Institutional Honor. In recognition to their outstanding professional work in wealth of the country and their institution. Circulate of Journalists of the Radio and Television.

B.      Selected peer-reviewed publications (in chronological order).

1. Ventosilla P. 1991.  Noix de coco contre moustiques.  Bol. Spore 33: 13, Juin. France.
2. Ventosilla P. 1991.  Mosquito control with bacteria grown in coconuts. Bol. OPS. 110: 528.
3. Ventosilla P. Snyder M. 1992. Methodology Guide.  Controlling Malaria. Lima: Inst. Med. Trop. Alexander von Humboldt-UPCH, International Development Research Centre (IDRC).  SAYWA Ed. Pp.1-29.
4. Ventosilla P., Marín D., Guerra H. 1994.  Mechanisms of action of Entomopatogenic Sporulated Bacilli. In: Talleres No 3.  Latin-American Workshop: Vector control. Universidad De Los Andes. Centro de Investigaciones “José Witremundo Torrealba”. Ed. Talleres Gráficos, Universidad de los Andes, Venezuela. pp. 131-133.
5. Birtles R. J., Canales J., Ventosilla P., Alvarez E., Harrison T.G., Guerra H., Llanos-Cuentas A., Raoult D. 1996. Prevalence and Genotypic analysis of Bartonella species isolated from intra-domicilliary animals in bartonellosis-endemic regions of Perú. In: Rickettsiae and Rickettsial Diseases. Proceedings of the Vth International Symposium. Stará Lesná, Sept. 1996. Slovac Republic. J. Kazár & R. Toman, Eds. Slovac Academic of  Sciences, Institute of Virology, Bratislava, Slovac Republic. Pp. 611-621.
6. Ventosilla P., 1996.  Biologic control of tropical diseases vectors. Boletin del Instituto Nacional de Salud. Año 2 ( 3): 56-58.
7. Ventosilla P., Guerra H., 1997.  Pilot production using whole coconuts and application in the field of Bacillus thuringiensis var. israelensis for biological control of Anopheles in malaria endemic areas in Perú.  Revista de Medicina Experimental del Instituto Nacional de Salud.  Segunda Epoca.  Vol XIV, No 2, July – December, Lima, Perú. pp 61.

8.        Birtles R., Canales J., Ventosilla P., Alvarez E., Guerra H., Llanos-Cuentas A., Raoult D., Doshi N., Harrison T.  1999.  Survey of Bartonella species infecting intra-domicilliary animals in the Huayllacallán Valley, Ancash, Perú, a region endemic for human bartonellosis. Am J Trop Med Hyg.  60: 799-805.

9. Montoya M., Maguiña C., Vigo.B., Caparó R., Briceño E., Astorga L, Ventosilla P., Pérez E., Guerra H., 1998.  Epidemic outbreak of Carrión’s Disease in the Sacred Valley of the Incas (Cusco).  Bol. Soc. Per. Med. Int.  11: 170-176.
10. Ventosilla P., Marín D., Guerra H., 1998.  Mechanisms of action of entomopatogenic bacilli.  Accepted by Revista de Medicina  Experimental del Instituto Nacional de Salud.  Segunda Epoca. Lima, Perú.

11.     Huarcaya E., Maguiña C., Ventosilla P., Merello J., Birtles R., Tello A. 2000. Cat scratch disease, the first prospective study in Lima, Perú . Folia Dermatológica. 11(1):21-29.

12.     Haurcaya E, Ventosilla P., Maguiña C., Merello J., Infante, B., Cox J., Guerra H., Tello A., Roult D. and Birtles R. 2001. First report of Bartonella henselae and B. clarrigeiae in patients with Cat scratch diseases and Bacillary angiomatosis in Lima. Diagnostico. Vol 40:01, pp. 13-19.

13.     Maguiña C., Henríquez C., Hinojosa J.C., Ventosilla P ., Infante B., Merello J. 2001. Reporte de un caso inusual de bacteremia persistente por Bartonella bacilliformis en un paciente  esplenectomizado y estudios de PCR y Western Blot. Revista Peruana de Enfermedades Infecciosas y Tropicales. Vol.1:04,  pp. 184-189.

14. Maguiña C., Sanchez E.; Gotuzzo E. ; Guerra H, Ventosilla P.,  Pérez E.. 2001. Estudios de nuevas zonas  endémicas de bartonellosis humana o enfermedad de Carrión en el Perú. Acta Medica Peruana XVIII: 22-27.
15. Henríquez, C; Infante, B; Merello, J; Gallino, M; Santivañez, L; Maguiña, C; Guerra, H; Birtles, R; Ventosilla, P. 2002. Identificación de Bartonella bacilliformis por métodos moleculares. Rev Med  Hered 13 (2):58-63.
16. Huarcaya, E; Infante, B; Chaparro, E; Merello, J; Ventosilla, P. 2002.  Linfadenomegalia cervical por Mycobacterium kansasii en un paciente inmunocompetente. Rev Soc Peruana Med Int 15(2):104-109.
17. Huarcaya, E; Maguiña, C; Merello, J; Cok, J; Birtles, R; Infante, B; Vidal, J; Tello, A; Ventosilla, P. 2002. A prospective study of cat-scratch disease in Lima-Peru. Rev Inst Med Trop S. Paulo 44(6):325- 330, Nov./Dec.
18. Birtles R., Fry N, Ventosilla P, Cáceres A , Sánchez E, Vizcarra H, and Raoult D. 2002. Identification of Bartonella bacilliformis Genotypes and Their Relevance to Epidemiological Investigations of Human  Bartonellosis. J Clin Microbiol 40 (10): 3606–, Oct.
19. Huarcaya E, Infante B, Maguiña C, Merello J, Birtles R, Ventosilla P. 2003. Identificación de Bartonella  spp. mediante reacción en cadena de la polimerasa y métodos microbiológicos. Diagnóstico, 2003;  43(4): 513-516.
20. Rolain JM, Novelli S, Ventosilla P, Maguina C, Guerra H, Raoult D. 2003. Immunofluorescence detection of Bartonella bacilliformis flagella in vitro and in vivo in human red blood cells as viewed by  laser confocal microscopy. Ann N Y Acad Sci. 990:581-4.
21. Huarcaya E., Infante B., Maguiña C., MerelloJ. Birtles R., Ventosilla P.  2003. Identificación de Bartonella sp. mediante reacción en cadena de la Polimerasa y métodos microbiológicos. Diagnóstico,  43(4): 513-516.
22. Henriquez C, Hinojosa J, Ventosilla P, Infante B, Merello J, Mallqui V, Verastegui M, Maguiña C. 2004. Report of an unusual case of persistent bacteremia by Bartonella bacilliformis in a splenectomized  patient. AJTMH. 2004 Jul;71(1):53-55. In: http://www.ajtmh.org/cgi/reprint/71/1/53

C.      Research Support. (Last three years).

Ongoing Research Support

1.        Study of the Immune Answer to the infection for Bartonella bacilliformis using Western Blot, ELISA and Dot-Elisa. Role: PI. Founded by: ITM-DGCD-Belgium Software for Mosquito larvae counter. Role: PI

2.        Characterization of immunoglobulines from patients with Bartonelosis. Role: PI.

3.        Pilot Project: Immunological Commitment in patient with human Bartonelosis. Funded by Inst. Hipólito Hunanue. Role : Co-investigator.

4.        Develop of a natural larvicida from Piper aduncum L. (" Matico ") to vector Control of Malaria, dengue and Yellow Fever. Founded by: Fondo Concursable Para apoyo a la Investigación U.P.C.H. Role : Co-investigator..

Completed Research Support

1. Evaluation of VectoBac (Bacillus thuringiensis isralensis H-14)  and Temephos for the control of larvae of An. albimanus, An. pseudopunctipennis, and Culex quinquefaciatus in Peru. 2002-2003. The aim of this study was to evaluate and compare the efficacy and residual activity of VectoBac WDG and Temephos again An. pseudopunctipennis, and Culex quinquefaciatus, in the laboratory and artificial ponds; and An. albimanus in rice fields. The LC₅₀ of Vectobac WDG against An. pseudopunctipennis was 0.143 mg/l and the LC₉₅ was 0.491 mg/l. The LC₅₀ for Temephos against An. pseudopunctipennis was 0.691 mg/l and the LC₉₅ was 3.128 mg/l. The LC₅₀ for Temephos against Cx. quinquefasciatus was 0.092 mg/l and the LC₉₅ was 0.245 mg/l. The LC₅₀ for Vectobac WDG against Cx. quinquefasciatus was 0.008 mg/l and the LC₉₅ was 0.018 mg/l. In artificial ponds, effect of Vectobac WDG against An. pseudopunctipennis was 21.1% - 54.1 % until day 4.5. The larvicide effect of Temephos was > 88%. For Cx. quinquefasciatus, Vectobac WDG showed mortalities from 98.5% to 99.5% until day 7. The effect of Temephos ranged from 94.5% to 99.5%. In rice fields, Vectobac significantly decreased the larval density of An. albimanus (P < 0.001) and for Temephos (P< 0.001).The reduction of the larval population using the Mulla´s formula for Vectobac WDG was 99.9 % after 24h and 75.0% at the end of the assays. Temephos reduced the larval population 97.5% over the same period. Founded by WHO.

Role: PI

2. Evaluation of knowledge and attitudes to the communities of the District Salitral-Sullana, Piura,  Perú, after six years of education (1992-1998) in biological control of malaria. 2004.  In Peru, the Health Ministry implements a Malaria Control Program that  provides patient treatment and controls the vector using insecticides. We developed a biological control method (Founded by IRDC ) for malaria using coconut-produced Bacillus thuringiensis Subsp. israelensis-H 14 (Bti) also on other methods of vector control (1992-1998). The goal for this project was to determine the knowledge, attitudes and practices on malaria and dengue control in Salitral after 6 years of the original intervention and Querecotillo without intervention.  We found that Salitral had more knowledge than Querecotillo on Bti (20.8%, CI 95%: 16.8  to 24.8 %); on methods of malaria control using personal protection and environmental management (11.8 %, CI 95%: 6.2 to 17.4%). The differences between Querecotillo and Salitral as regards their attitudes and practices was 12.3%(CI 95%: 8.1-16.5). In conclusion, the transfer of knowledge, competencies and abilities for malaria and dengue control to the community of Salitral has become an important facilitator for the implementation of local community-wide activities oriented to disease control. Salitral has better knowledge, attitudes and practices, as a consequence, has had much less malaria (1998 = 327, 2000 = 108, 2002 = 301, 2003 = 34, 2004 =  0, 2005= 0 cases) and no dengue since dengue arrived in Peru, while Querecotillo has had cases every year (2001= 836, 2002 = 13, 2003 = 23 cases). Supported by CONCYTEC Role: PI.

3. Influence of climatic variability on the malaria incidence due to plasmodium falciparum and plasmodium vivax in a peruvian endemic area.

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24/10/2005